The Magellan spacecraft was deployed from the shuttle's payload bay six hours and 14 minutes into the mission.[5] Two successive IUS propulsion burns placed the spacecraft on its trajectory to Venus about an hour later. Magellan arrived at Venus in August 1990 and began a 243-day mission of mapping the planet's surface with radar.

Three mid-deck experiments were included on the mission. All had flown before. Mission Specialist Cleave used a portable laptop computer to operate and monitor the Fluids Experiment Apparatus (FEA).[5] An 8-millimeter video camcorder, flown for the first time on the Shuttle, provided the opportunity for the crew to record and downlink on-orbit activities such as the FEA, which was a joint endeavor between Rockwell International and NASA. Payload bay video cameras were used to record storm systems from orbit as part of the Mesoscale Lightning Experiment.